Construct: shRNA TRCN0000143861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175902.3-560s1c1
DNA Barcode:
CACTTTGTGAACCTGTACAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OGFOD3 (79701)
Gene Description:
2-oxoglutarate and iron dependent oxygenase domain containing 3
Transcript:
RefSeq NM_175902.3 (NON-CURRENT)
Match location:
Position 560 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTTGTGAACCTGTACAGA
Hairpin Sequence:
5'-CCGG-CACTTTGTGAACCTGTACAGA-CTCGAG-TCTGTACAGGTTCACAAAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTTGTGAACCTGTACAGACTCGAGTCTGTACAGGTTCACAAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTTGTGAACCTGTACAGACTCGAGTCTGTACAGGTTCACAAAGTG-3'

Other clones with same target sequence:

(none)