Construct: shRNA TRCN0000143862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178124.3-1056s1c1
DNA Barcode:
CCAGCTAAATCATGACACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CXorf40A (91966)
Gene Description:
chromosome X open reading frame 40A
Transcript:
RefSeq NM_178124.3 (NON-CURRENT)
Match location:
Position 1056 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTAAATCATGACACCTT
Hairpin Sequence:
5'-CCGG-CCAGCTAAATCATGACACCTT-CTCGAG-AAGGTGTCATGATTTAGCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTAAATCATGACACCTTCTCGAGAAGGTGTCATGATTTAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTAAATCATGACACCTTCTCGAGAAGGTGTCATGATTTAGCTGG-3'

Other clones with same target sequence:

(none)