Construct: shRNA TRCN0000143866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199167.1-1108s1c1
DNA Barcode:
CGATCAGGTTGGTCAATGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLUL1 (27098)
Gene Description:
clusterin like 1
Transcript:
RefSeq NM_199167.1 (CURRENT)
Match location:
Position 1108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCAGGTTGGTCAATGTAT
Hairpin Sequence:
5'-CCGG-CGATCAGGTTGGTCAATGTAT-CTCGAG-ATACATTGACCAACCTGATCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCAGGTTGGTCAATGTATCTCGAGATACATTGACCAACCTGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCAGGTTGGTCAATGTATCTCGAGATACATTGACCAACCTGATCG-3'

Other clones with same target sequence:

(none)