Construct: shRNA TRCN0000143893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152675.1-1327s1c1
DNA Barcode:
CCAGACTTTAATGTTCCTCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC57 (284001)
Gene Description:
coiled-coil domain containing 57
Transcript:
RefSeq NM_152675.1 (NON-CURRENT)
Match location:
Position 1327 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACTTTAATGTTCCTCCA
Hairpin Sequence:
5'-CCGG-CCAGACTTTAATGTTCCTCCA-CTCGAG-TGGAGGAACATTAAAGTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACTTTAATGTTCCTCCACTCGAGTGGAGGAACATTAAAGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACTTTAATGTTCCTCCACTCGAGTGGAGGAACATTAAAGTCTGG-3'

Other clones with same target sequence:

(none)