Construct: shRNA TRCN0000143961

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014924.3-2431s1c1
DNA Barcode:
CCACTTTCTTTCTATGGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG14 (22863)
Gene Description:
autophagy related 14
Transcript:
RefSeq NM_014924.3 (NON-CURRENT)
Match location:
Position 2431 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143961)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTTCTTTCTATGGGATT
Hairpin Sequence:
5'-CCGG-CCACTTTCTTTCTATGGGATT-CTCGAG-AATCCCATAGAAAGAAAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTTCTTTCTATGGGATTCTCGAGAATCCCATAGAAAGAAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTTCTTTCTATGGGATTCTCGAGAATCCCATAGAAAGAAAGTGG-3'

Other clones with same target sequence:

(none)