Construct: shRNA TRCN0000143992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152748.2-2764s1c1
DNA Barcode:
CAAAGAAACCTGCTCTAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA1324L (222223)
Gene Description:
KIAA1324 like
Transcript:
RefSeq NM_152748.2 (NON-CURRENT)
Match location:
Position 2764 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000143992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGAAACCTGCTCTAGTTT
Hairpin Sequence:
5'-CCGG-CAAAGAAACCTGCTCTAGTTT-CTCGAG-AAACTAGAGCAGGTTTCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGAAACCTGCTCTAGTTTCTCGAGAAACTAGAGCAGGTTTCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGAAACCTGCTCTAGTTTCTCGAGAAACTAGAGCAGGTTTCTTTG-3'

Other clones with same target sequence:

(none)