Construct: shRNA TRCN0000144014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005862.1-2402s1c1
DNA Barcode:
CGATTCAATCATTCTGTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAG1 (10274)
Gene Description:
stromal antigen 1
Transcript:
RefSeq NM_005862.1 (NON-CURRENT)
Match location:
Position 2402 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATTCAATCATTCTGTGGAA
Hairpin Sequence:
5'-CCGG-CGATTCAATCATTCTGTGGAA-CTCGAG-TTCCACAGAATGATTGAATCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATTCAATCATTCTGTGGAACTCGAGTTCCACAGAATGATTGAATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATTCAATCATTCTGTGGAACTCGAGTTCCACAGAATGATTGAATCG-3'

Other clones with same target sequence:

(none)