Construct: shRNA TRCN0000144068

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145018.2-3301s1c1
DNA Barcode:
CTTGGAGAGAAGCAAATTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDIAS (220042)
Gene Description:
DNA damage induced apoptosis suppressor
Transcript:
RefSeq NM_145018.2 (NON-CURRENT)
Match location:
Position 3301 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144068)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGAGAGAAGCAAATTGAA
Hairpin Sequence:
5'-CCGG-CTTGGAGAGAAGCAAATTGAA-CTCGAG-TTCAATTTGCTTCTCTCCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGAGAGAAGCAAATTGAACTCGAGTTCAATTTGCTTCTCTCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGAGAGAAGCAAATTGAACTCGAGTTCAATTTGCTTCTCTCCAAG-3'

Other clones with same target sequence:

(none)