Construct: shRNA TRCN0000144099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004320.1-1941s1c1
DNA Barcode:
CAGTTACCTTTGCAATCCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGMO (392636)
Gene Description:
alkylglycerol monooxygenase
Transcript:
RefSeq NM_001004320.1 (NON-CURRENT)
Match location:
Position 1941 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTACCTTTGCAATCCATA
Hairpin Sequence:
5'-CCGG-CAGTTACCTTTGCAATCCATA-CTCGAG-TATGGATTGCAAAGGTAACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTACCTTTGCAATCCATACTCGAGTATGGATTGCAAAGGTAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTACCTTTGCAATCCATACTCGAGTATGGATTGCAAAGGTAACTG-3'

Other clones with same target sequence:

(none)