Construct: shRNA TRCN0000144163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138786.1-259s1c1
DNA Barcode:
CAGCAATAAACTCACCAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TM4SF18 (116441)
Gene Description:
transmembrane 4 L six family member 18
Transcript:
RefSeq NM_138786.1 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAATAAACTCACCAACTA
Hairpin Sequence:
5'-CCGG-CAGCAATAAACTCACCAACTA-CTCGAG-TAGTTGGTGAGTTTATTGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAATAAACTCACCAACTACTCGAGTAGTTGGTGAGTTTATTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAATAAACTCACCAACTACTCGAGTAGTTGGTGAGTTTATTGCTG-3'

Other clones with same target sequence:

(none)