Construct: shRNA TRCN0000144290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015469.1-1357s1c1
DNA Barcode:
CCACATTGTCATTTGTGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NIPSNAP3A (25934)
Gene Description:
nipsnap homolog 3A
Transcript:
RefSeq NM_015469.1 (NON-CURRENT)
Match location:
Position 1357 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATTGTCATTTGTGACAT
Hairpin Sequence:
5'-CCGG-CCACATTGTCATTTGTGACAT-CTCGAG-ATGTCACAAATGACAATGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATTGTCATTTGTGACATCTCGAGATGTCACAAATGACAATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATTGTCATTTGTGACATCTCGAGATGTCACAAATGACAATGTGG-3'

Other clones with same target sequence:

(none)