Construct: shRNA TRCN0000144318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152352.1-1403s1c1
DNA Barcode:
CCCAGTTTAAGAATTTGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM210A (125228)
Gene Description:
family with sequence similarity 210 member A
Transcript:
RefSeq NM_152352.1 (NON-CURRENT)
Match location:
Position 1403 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTTTAAGAATTTGCCAT
Hairpin Sequence:
5'-CCGG-CCCAGTTTAAGAATTTGCCAT-CTCGAG-ATGGCAAATTCTTAAACTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTTTAAGAATTTGCCATCTCGAGATGGCAAATTCTTAAACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTTTAAGAATTTGCCATCTCGAGATGGCAAATTCTTAAACTGGG-3'

Other clones with same target sequence:

(none)