Construct: shRNA TRCN0000144359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013401.2-1864s1c1
DNA Barcode:
CCTTGTTAAGATGAGTCAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB3IL1 (5866)
Gene Description:
RAB3A interacting protein like 1
Transcript:
RefSeq NM_013401.2 (NON-CURRENT)
Match location:
Position 1864 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTTAAGATGAGTCAAGA
Hairpin Sequence:
5'-CCGG-CCTTGTTAAGATGAGTCAAGA-CTCGAG-TCTTGACTCATCTTAACAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTTAAGATGAGTCAAGACTCGAGTCTTGACTCATCTTAACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTTAAGATGAGTCAAGACTCGAGTCTTGACTCATCTTAACAAGG-3'

Other clones with same target sequence:

(none)