Construct: shRNA TRCN0000144376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021049.3-1274s1c1
DNA Barcode:
CAACATTCTTAGTAGTGGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA5 (4104)
Gene Description:
MAGE family member A5
Transcript:
RefSeq NM_021049.3 (NON-CURRENT)
Match location:
Position 1274 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACATTCTTAGTAGTGGGTT
Hairpin Sequence:
5'-CCGG-CAACATTCTTAGTAGTGGGTT-CTCGAG-AACCCACTACTAAGAATGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACATTCTTAGTAGTGGGTTCTCGAGAACCCACTACTAAGAATGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACATTCTTAGTAGTGGGTTCTCGAGAACCCACTACTAAGAATGTTG-3'

Other clones with same target sequence:

(none)