Construct: shRNA TRCN0000144531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173599.1-326s1c1
DNA Barcode:
CAAAGACTAAGTAGCAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C12orf40 (283461)
Gene Description:
chromosome 12 open reading frame 40
Transcript:
RefSeq NM_173599.1 (NON-CURRENT)
Match location:
Position 326 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGACTAAGTAGCAAGGAA
Hairpin Sequence:
5'-CCGG-CAAAGACTAAGTAGCAAGGAA-CTCGAG-TTCCTTGCTACTTAGTCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGACTAAGTAGCAAGGAACTCGAGTTCCTTGCTACTTAGTCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGACTAAGTAGCAAGGAACTCGAGTTCCTTGCTACTTAGTCTTTG-3'

Other clones with same target sequence:

(none)