Construct: shRNA TRCN0000144533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182496.1-324s1c1
DNA Barcode:
CAGAGATCTCTTTCTTGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC38 (120935)
Gene Description:
coiled-coil domain containing 38
Transcript:
RefSeq NM_182496.1 (NON-CURRENT)
Match location:
Position 324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGATCTCTTTCTTGTCAA
Hairpin Sequence:
5'-CCGG-CAGAGATCTCTTTCTTGTCAA-CTCGAG-TTGACAAGAAAGAGATCTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGATCTCTTTCTTGTCAACTCGAGTTGACAAGAAAGAGATCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGATCTCTTTCTTGTCAACTCGAGTTGACAAGAAAGAGATCTCTG-3'

Other clones with same target sequence:

(none)