Construct: shRNA TRCN0000144561

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020467.2-645s1c1
DNA Barcode:
GACATGGAGAAAGCTGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMAGP (57228)
Gene Description:
small cell adhesion glycoprotein
Transcript:
RefSeq NM_020467.2 (NON-CURRENT)
Match location:
Position 645 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144561)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATGGAGAAAGCTGTTTAT
Hairpin Sequence:
5'-CCGG-GACATGGAGAAAGCTGTTTAT-CTCGAG-ATAAACAGCTTTCTCCATGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATGGAGAAAGCTGTTTATCTCGAGATAAACAGCTTTCTCCATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATGGAGAAAGCTGTTTATCTCGAGATAAACAGCTTTCTCCATGTC-3'

Other clones with same target sequence:

  1. TRCN0000276305