Construct: shRNA TRCN0000144753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021120.1-2568s1c1
DNA Barcode:
GAGTACTTTACAGCCATTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG3 (1741)
Gene Description:
discs large MAGUK scaffold protein 3
Transcript:
RefSeq NM_021120.1 (NON-CURRENT)
Match location:
Position 2568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTACTTTACAGCCATTGTA
Hairpin Sequence:
5'-CCGG-GAGTACTTTACAGCCATTGTA-CTCGAG-TACAATGGCTGTAAAGTACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTACTTTACAGCCATTGTACTCGAGTACAATGGCTGTAAAGTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTACTTTACAGCCATTGTACTCGAGTACAATGGCTGTAAAGTACTC-3'

Other clones with same target sequence:

  1. TRCN0000330107