Construct: shRNA TRCN0000144798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016618.1-1151s1c1
DNA Barcode:
GACCAGTCTATTCTTGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRCC1 (51315)
Gene Description:
lysine rich coiled-coil 1
Transcript:
RefSeq NM_016618.1 (NON-CURRENT)
Match location:
Position 1151 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAGTCTATTCTTGGATTT
Hairpin Sequence:
5'-CCGG-GACCAGTCTATTCTTGGATTT-CTCGAG-AAATCCAAGAATAGACTGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAGTCTATTCTTGGATTTCTCGAGAAATCCAAGAATAGACTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAGTCTATTCTTGGATTTCTCGAGAAATCCAAGAATAGACTGGTC-3'

Other clones with same target sequence:

  1. TRCN0000343768
  2. TRCN0000216463