Construct: shRNA TRCN0000144831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018224.1-438s1c1
DNA Barcode:
GTTGATGCCAAGTTGAAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COA1 (55744)
Gene Description:
cytochrome c oxidase assembly factor 1 homolog
Transcript:
RefSeq NM_018224.1 (NON-CURRENT)
Match location:
Position 438 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGATGCCAAGTTGAAGATT
Hairpin Sequence:
5'-CCGG-GTTGATGCCAAGTTGAAGATT-CTCGAG-AATCTTCAACTTGGCATCAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGATGCCAAGTTGAAGATTCTCGAGAATCTTCAACTTGGCATCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGATGCCAAGTTGAAGATTCTCGAGAATCTTCAACTTGGCATCAAC-3'

Other clones with same target sequence:

(none)