Construct: shRNA TRCN0000144845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017435.1-461s1c1
DNA Barcode:
GATTGATGACTTCACTGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CT45A3 (441519)
Gene Description:
cancer/testis antigen family 45 member A3
Transcript:
RefSeq NM_001017435.1 (NON-CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGATGACTTCACTGGTTT
Hairpin Sequence:
5'-CCGG-GATTGATGACTTCACTGGTTT-CTCGAG-AAACCAGTGAAGTCATCAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGATGACTTCACTGGTTTCTCGAGAAACCAGTGAAGTCATCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGATGACTTCACTGGTTTCTCGAGAAACCAGTGAAGTCATCAATC-3'

Other clones with same target sequence:

  1. TRCN0000147521
  2. TRCN0000158798