Construct: shRNA TRCN0000144855
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_017935.2-1391s1c1
- DNA Barcode:
- GAAGAGGATATTGCCTCATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BANK1 (55024)
- Gene Description:
- B cell scaffold protein with ankyrin repeats 1
- Transcript:
- RefSeq NM_017935.2 (NON-CURRENT)
- Match location:
- Position 1391 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000144855)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGAGGATATTGCCTCATTT
- Hairpin Sequence:
-
5'-CCGG-GAAGAGGATATTGCCTCATTT-CTCGAG-AAATGAGGCAATATCCTCTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGAGGATATTGCCTCATTTCTCGAGAAATGAGGCAATATCCTCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGAGGATATTGCCTCATTTCTCGAGAAATGAGGCAATATCCTCTTC-3'
Other clones with same target sequence:
(none)
