Construct: shRNA TRCN0000144910

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020704.1-1611s1c1
DNA Barcode:
GCTAAGACAGACTCTATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STRIP2 (57464)
Gene Description:
striatin interacting protein 2
Transcript:
RefSeq NM_020704.1 (NON-CURRENT)
Match location:
Position 1611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144910)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAGACAGACTCTATCAAT
Hairpin Sequence:
5'-CCGG-GCTAAGACAGACTCTATCAAT-CTCGAG-ATTGATAGAGTCTGTCTTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAGACAGACTCTATCAATCTCGAGATTGATAGAGTCTGTCTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAGACAGACTCTATCAATCTCGAGATTGATAGAGTCTGTCTTAGC-3'

Other clones with same target sequence:

(none)