Construct: shRNA TRCN0000144938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024820.1-1453s1c1
DNA Barcode:
GAAGTGGAGCAATTCTGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DENND1A (57706)
Gene Description:
DENN domain containing 1A
Transcript:
RefSeq NM_024820.1 (NON-CURRENT)
Match location:
Position 1453 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGGAGCAATTCTGAATA
Hairpin Sequence:
5'-CCGG-GAAGTGGAGCAATTCTGAATA-CTCGAG-TATTCAGAATTGCTCCACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGGAGCAATTCTGAATACTCGAGTATTCAGAATTGCTCCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGGAGCAATTCTGAATACTCGAGTATTCAGAATTGCTCCACTTC-3'

Other clones with same target sequence:

(none)