Construct: shRNA TRCN0000144984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023071.1-2631s1c1
DNA Barcode:
GCCTTGTTTCCTATTACCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPATS2 (65244)
Gene Description:
spermatogenesis associated serine rich 2
Transcript:
RefSeq NM_023071.1 (NON-CURRENT)
Match location:
Position 2631 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000144984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGTTTCCTATTACCTTA
Hairpin Sequence:
5'-CCGG-GCCTTGTTTCCTATTACCTTA-CTCGAG-TAAGGTAATAGGAAACAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGTTTCCTATTACCTTACTCGAGTAAGGTAATAGGAAACAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGTTTCCTATTACCTTACTCGAGTAAGGTAATAGGAAACAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000281053