Construct: shRNA TRCN0000145126

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014929.2-323s1c1
DNA Barcode:
GCTTTCAAACAAAGGGCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FASTKD2 (22868)
Gene Description:
FAST kinase domains 2
Transcript:
RefSeq NM_014929.2 (NON-CURRENT)
Match location:
Position 323 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145126)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCAAACAAAGGGCATAA
Hairpin Sequence:
5'-CCGG-GCTTTCAAACAAAGGGCATAA-CTCGAG-TTATGCCCTTTGTTTGAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCAAACAAAGGGCATAACTCGAGTTATGCCCTTTGTTTGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCAAACAAAGGGCATAACTCGAGTTATGCCCTTTGTTTGAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000343506