Construct: shRNA TRCN0000145220

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015474.2-2048s1c1
DNA Barcode:
GCACAATTCATTTAGAGGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMHD1 (25939)
Gene Description:
SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
Transcript:
RefSeq NM_015474.2 (NON-CURRENT)
Match location:
Position 2048 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145220)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAATTCATTTAGAGGCTT
Hairpin Sequence:
5'-CCGG-GCACAATTCATTTAGAGGCTT-CTCGAG-AAGCCTCTAAATGAATTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAATTCATTTAGAGGCTTCTCGAGAAGCCTCTAAATGAATTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAATTCATTTAGAGGCTTCTCGAGAAGCCTCTAAATGAATTGTGC-3'

Other clones with same target sequence:

(none)