Construct: shRNA TRCN0000145237

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145018.2-807s1c1
DNA Barcode:
GCACCTAACAATCACTTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDIAS (220042)
Gene Description:
DNA damage induced apoptosis suppressor
Transcript:
RefSeq NM_145018.2 (NON-CURRENT)
Match location:
Position 807 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145237)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTAACAATCACTTACTT
Hairpin Sequence:
5'-CCGG-GCACCTAACAATCACTTACTT-CTCGAG-AAGTAAGTGATTGTTAGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTAACAATCACTTACTTCTCGAGAAGTAAGTGATTGTTAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTAACAATCACTTACTTCTCGAGAAGTAAGTGATTGTTAGGTGC-3'

Other clones with same target sequence:

(none)