Construct: shRNA TRCN0000145266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_205849.1-408s1c1
DNA Barcode:
GTGATGAATGTGAGGAAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM9B (171483)
Gene Description:
family with sequence similarity 9 member B
Transcript:
RefSeq NM_205849.1 (NON-CURRENT)
Match location:
Position 408 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATGAATGTGAGGAAAGAA
Hairpin Sequence:
5'-CCGG-GTGATGAATGTGAGGAAAGAA-CTCGAG-TTCTTTCCTCACATTCATCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATGAATGTGAGGAAAGAACTCGAGTTCTTTCCTCACATTCATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATGAATGTGAGGAAAGAACTCGAGTTCTTTCCTCACATTCATCAC-3'

Other clones with same target sequence:

(none)