Construct: shRNA TRCN0000145289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024735.2-680s1c1
DNA Barcode:
GATTGTGAAGAAGGATGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO31 (79791)
Gene Description:
F-box protein 31
Transcript:
RefSeq NM_024735.2 (NON-CURRENT)
Match location:
Position 680 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGTGAAGAAGGATGAGTT
Hairpin Sequence:
5'-CCGG-GATTGTGAAGAAGGATGAGTT-CTCGAG-AACTCATCCTTCTTCACAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGTGAAGAAGGATGAGTTCTCGAGAACTCATCCTTCTTCACAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGTGAAGAAGGATGAGTTCTCGAGAACTCATCCTTCTTCACAATC-3'

Other clones with same target sequence:

(none)