Construct: shRNA TRCN0000145303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002540.3-1096s1c1
DNA Barcode:
CTTCAGAAGAAACACCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ODF2 (4957)
Gene Description:
outer dense fiber of sperm tails 2
Transcript:
RefSeq NM_002540.3 (NON-CURRENT)
Match location:
Position 1096 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGAAGAAACACCTACAA
Hairpin Sequence:
5'-CCGG-CTTCAGAAGAAACACCTACAA-CTCGAG-TTGTAGGTGTTTCTTCTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGAAGAAACACCTACAACTCGAGTTGTAGGTGTTTCTTCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGAAGAAACACCTACAACTCGAGTTGTAGGTGTTTCTTCTGAAG-3'

Other clones with same target sequence:

  1. TRCN0000280594