Construct: shRNA TRCN0000145359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058168.2-151s1c1
DNA Barcode:
GAACTCAAGGAGGCTATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCAT4 (118425)
Gene Description:
prostate cancer associated transcript 4
Transcript:
RefSeq NM_058168.2 (NON-CURRENT)
Match location:
Position 151 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTCAAGGAGGCTATTTAT
Hairpin Sequence:
5'-CCGG-GAACTCAAGGAGGCTATTTAT-CTCGAG-ATAAATAGCCTCCTTGAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTCAAGGAGGCTATTTATCTCGAGATAAATAGCCTCCTTGAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTCAAGGAGGCTATTTATCTCGAGATAAATAGCCTCCTTGAGTTC-3'

Other clones with same target sequence:

(none)