Construct: shRNA TRCN0000145399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174921.1-1164s1c1
DNA Barcode:
GCAGATGATTTACAGTCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMIM14 (201895)
Gene Description:
small integral membrane protein 14
Transcript:
RefSeq NM_174921.1 (NON-CURRENT)
Match location:
Position 1164 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGATTTACAGTCCATT
Hairpin Sequence:
5'-CCGG-GCAGATGATTTACAGTCCATT-CTCGAG-AATGGACTGTAAATCATCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGATTTACAGTCCATTCTCGAGAATGGACTGTAAATCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGATTTACAGTCCATTCTCGAGAATGGACTGTAAATCATCTGC-3'

Other clones with same target sequence:

(none)