Construct: shRNA TRCN0000145404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004623.2-210s1c1
DNA Barcode:
GCTTGTCTCCAGTCAATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTC4 (7268)
Gene Description:
tetratricopeptide repeat domain 4
Transcript:
RefSeq NM_004623.2 (NON-CURRENT)
Match location:
Position 210 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTCTCCAGTCAATTATT
Hairpin Sequence:
5'-CCGG-GCTTGTCTCCAGTCAATTATT-CTCGAG-AATAATTGACTGGAGACAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTCTCCAGTCAATTATTCTCGAGAATAATTGACTGGAGACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTCTCCAGTCAATTATTCTCGAGAATAATTGACTGGAGACAAGC-3'

Other clones with same target sequence:

(none)