Construct: shRNA TRCN0000145433

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014678.2-977s1c1
DNA Barcode:
GATGAAGATAGGCAGTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP6R2 (9701)
Gene Description:
protein phosphatase 6 regulatory subunit 2
Transcript:
RefSeq NM_014678.2 (NON-CURRENT)
Match location:
Position 977 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145433)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAAGATAGGCAGTCAAAT
Hairpin Sequence:
5'-CCGG-GATGAAGATAGGCAGTCAAAT-CTCGAG-ATTTGACTGCCTATCTTCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAAGATAGGCAGTCAAATCTCGAGATTTGACTGCCTATCTTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAAGATAGGCAGTCAAATCTCGAGATTTGACTGCCTATCTTCATC-3'

Other clones with same target sequence:

(none)