Construct: shRNA TRCN0000145452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144713.1-1583s1c1
DNA Barcode:
GATACTGCTATACTGTCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RMDN2 (151393)
Gene Description:
regulator of microtubule dynamics 2
Transcript:
RefSeq NM_144713.1 (NON-CURRENT)
Match location:
Position 1583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACTGCTATACTGTCTCAA
Hairpin Sequence:
5'-CCGG-GATACTGCTATACTGTCTCAA-CTCGAG-TTGAGACAGTATAGCAGTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACTGCTATACTGTCTCAACTCGAGTTGAGACAGTATAGCAGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACTGCTATACTGTCTCAACTCGAGTTGAGACAGTATAGCAGTATC-3'

Other clones with same target sequence:

(none)