Construct: shRNA TRCN0000145512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198530.2-745s1c1
DNA Barcode:
GTAGAGATGTTCATGTCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MXRA7 (439921)
Gene Description:
matrix remodeling associated 7
Transcript:
RefSeq NM_198530.2 (NON-CURRENT)
Match location:
Position 745 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGAGATGTTCATGTCTGTT
Hairpin Sequence:
5'-CCGG-GTAGAGATGTTCATGTCTGTT-CTCGAG-AACAGACATGAACATCTCTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGAGATGTTCATGTCTGTTCTCGAGAACAGACATGAACATCTCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGAGATGTTCATGTCTGTTCTCGAGAACAGACATGAACATCTCTAC-3'

Other clones with same target sequence:

(none)