Construct: shRNA TRCN0000145569

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015522.2-938s1c1
DNA Barcode:
GACAAAGACTAGATGAAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYNC2LI1 (51626)
Gene Description:
dynein cytoplasmic 2 light intermediate chain 1
Transcript:
RefSeq NM_015522.2 (NON-CURRENT)
Match location:
Position 938 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145569)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAAAGACTAGATGAAGCTA
Hairpin Sequence:
5'-CCGG-GACAAAGACTAGATGAAGCTA-CTCGAG-TAGCTTCATCTAGTCTTTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAAAGACTAGATGAAGCTACTCGAGTAGCTTCATCTAGTCTTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAAAGACTAGATGAAGCTACTCGAGTAGCTTCATCTAGTCTTTGTC-3'

Other clones with same target sequence:

(none)