Construct: shRNA TRCN0000145580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017658.2-1819s1c1
DNA Barcode:
CAAATGTGAACTCTCGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLHL28 (54813)
Gene Description:
kelch like family member 28
Transcript:
RefSeq NM_017658.2 (NON-CURRENT)
Match location:
Position 1819 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATGTGAACTCTCGGAAAT
Hairpin Sequence:
5'-CCGG-CAAATGTGAACTCTCGGAAAT-CTCGAG-ATTTCCGAGAGTTCACATTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATGTGAACTCTCGGAAATCTCGAGATTTCCGAGAGTTCACATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATGTGAACTCTCGGAAATCTCGAGATTTCCGAGAGTTCACATTTG-3'

Other clones with same target sequence:

(none)