Construct: shRNA TRCN0000145588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030814.4-5700s1c1
DNA Barcode:
CATGTAGGACTCTGAACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIR600HG (81571)
Gene Description:
MIR600 host gene
Transcript:
RefSeq NM_030814.4 (NON-CURRENT)
Match location:
Position 5700 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTAGGACTCTGAACTTTA
Hairpin Sequence:
5'-CCGG-CATGTAGGACTCTGAACTTTA-CTCGAG-TAAAGTTCAGAGTCCTACATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTAGGACTCTGAACTTTACTCGAGTAAAGTTCAGAGTCCTACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTAGGACTCTGAACTTTACTCGAGTAAAGTTCAGAGTCCTACATG-3'

Other clones with same target sequence:

(none)