Construct: shRNA TRCN0000145597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012417.1-496s1c1
DNA Barcode:
CGACTCGATCTGGAATTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MS4A13 (503497)
Gene Description:
membrane spanning 4-domains A13
Transcript:
RefSeq NM_001012417.1 (NON-CURRENT)
Match location:
Position 496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000145597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTCGATCTGGAATTATAA
Hairpin Sequence:
5'-CCGG-CGACTCGATCTGGAATTATAA-CTCGAG-TTATAATTCCAGATCGAGTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTCGATCTGGAATTATAACTCGAGTTATAATTCCAGATCGAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTCGATCTGGAATTATAACTCGAGTTATAATTCCAGATCGAGTCG-3'

Other clones with same target sequence:

(none)