Construct: shRNA TRCN0000146275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018688.3-915s1c1
DNA Barcode:
CCTTTGCAATGAATGACTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BIN3 (55909)
Gene Description:
bridging integrator 3
Transcript:
RefSeq NM_018688.3 (NON-CURRENT)
Match location:
Position 915 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGCAATGAATGACTCTT
Hairpin Sequence:
5'-CCGG-CCTTTGCAATGAATGACTCTT-CTCGAG-AAGAGTCATTCATTGCAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGCAATGAATGACTCTTCTCGAGAAGAGTCATTCATTGCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGCAATGAATGACTCTTCTCGAGAAGAGTCATTCATTGCAAAGG-3'

Other clones with same target sequence:

(none)