Construct: shRNA TRCN0000146281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033069.1-590s1c1
DNA Barcode:
CCAGTCAAGATTACAGATAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C6orf114 (85411)
Gene Description:
chromosome 6 open reading frame 114
Transcript:
RefSeq NM_033069.1 (NON-CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCAAGATTACAGATAGA
Hairpin Sequence:
5'-CCGG-CCAGTCAAGATTACAGATAGA-CTCGAG-TCTATCTGTAATCTTGACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCAAGATTACAGATAGACTCGAGTCTATCTGTAATCTTGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCAAGATTACAGATAGACTCGAGTCTATCTGTAATCTTGACTGG-3'

Other clones with same target sequence:

(none)