Construct: shRNA TRCN0000146335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020927.1-3176s1c1
DNA Barcode:
CCTAGTAAGTTGGGTAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAT1L (57687)
Gene Description:
vesicle amine transport 1 like
Transcript:
RefSeq NM_020927.1 (NON-CURRENT)
Match location:
Position 3176 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGTAAGTTGGGTAGAAAT
Hairpin Sequence:
5'-CCGG-CCTAGTAAGTTGGGTAGAAAT-CTCGAG-ATTTCTACCCAACTTACTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGTAAGTTGGGTAGAAATCTCGAGATTTCTACCCAACTTACTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGTAAGTTGGGTAGAAATCTCGAGATTTCTACCCAACTTACTAGG-3'

Other clones with same target sequence:

(none)