Construct: shRNA TRCN0000146367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004475.1-1136s1c1
DNA Barcode:
GAGACAACAGTAAGGTCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLOT2 (2319)
Gene Description:
flotillin 2
Transcript:
RefSeq NM_004475.1 (NON-CURRENT)
Match location:
Position 1136 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACAACAGTAAGGTCACAT
Hairpin Sequence:
5'-CCGG-GAGACAACAGTAAGGTCACAT-CTCGAG-ATGTGACCTTACTGTTGTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACAACAGTAAGGTCACATCTCGAGATGTGACCTTACTGTTGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACAACAGTAAGGTCACATCTCGAGATGTGACCTTACTGTTGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000280590