Construct: shRNA TRCN0000146371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015289.1-2288s1c1
DNA Barcode:
CCACAAACACTATGACCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS39 (23339)
Gene Description:
VPS39 subunit of HOPS complex
Transcript:
RefSeq NM_015289.1 (NON-CURRENT)
Match location:
Position 2288 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAAACACTATGACCGAAA
Hairpin Sequence:
5'-CCGG-CCACAAACACTATGACCGAAA-CTCGAG-TTTCGGTCATAGTGTTTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAAACACTATGACCGAAACTCGAGTTTCGGTCATAGTGTTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAAACACTATGACCGAAACTCGAGTTTCGGTCATAGTGTTTGTGG-3'

Other clones with same target sequence:

(none)