Construct: shRNA TRCN0000146394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138417.1-1038s1c1
DNA Barcode:
CAACTGGCCAACATGTTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KTI12 (112970)
Gene Description:
KTI12 chromatin associated homolog
Transcript:
RefSeq NM_138417.1 (NON-CURRENT)
Match location:
Position 1038 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTGGCCAACATGTTTCTT
Hairpin Sequence:
5'-CCGG-CAACTGGCCAACATGTTTCTT-CTCGAG-AAGAAACATGTTGGCCAGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTGGCCAACATGTTTCTTCTCGAGAAGAAACATGTTGGCCAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTGGCCAACATGTTTCTTCTCGAGAAGAAACATGTTGGCCAGTTG-3'

Other clones with same target sequence:

(none)