Construct: shRNA TRCN0000146429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199459.2-2738s1c1
DNA Barcode:
CTCACTTGTAAGCTCCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C10orf71 (118461)
Gene Description:
chromosome 10 open reading frame 71
Transcript:
RefSeq NM_199459.2 (NON-CURRENT)
Match location:
Position 2738 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACTTGTAAGCTCCTTGAT
Hairpin Sequence:
5'-CCGG-CTCACTTGTAAGCTCCTTGAT-CTCGAG-ATCAAGGAGCTTACAAGTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACTTGTAAGCTCCTTGATCTCGAGATCAAGGAGCTTACAAGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACTTGTAAGCTCCTTGATCTCGAGATCAAGGAGCTTACAAGTGAG-3'

Other clones with same target sequence:

(none)