Construct: shRNA TRCN0000146509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015681.2-240s1c1
DNA Barcode:
CTCTACTGCAAGTACTGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B9D1 (27077)
Gene Description:
B9 domain containing 1
Transcript:
RefSeq NM_015681.2 (NON-CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTACTGCAAGTACTGCTTT
Hairpin Sequence:
5'-CCGG-CTCTACTGCAAGTACTGCTTT-CTCGAG-AAAGCAGTACTTGCAGTAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTACTGCAAGTACTGCTTTCTCGAGAAAGCAGTACTTGCAGTAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTACTGCAAGTACTGCTTTCTCGAGAAAGCAGTACTTGCAGTAGAG-3'

Other clones with same target sequence:

  1. TRCN0000274987