Construct: shRNA TRCN0000146519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178230.1-390s1c1
DNA Barcode:
CAACACAAATGGTTCCCAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIAL4A (164022)
Gene Description:
peptidylprolyl isomerase A (cyclophilin A)-like 4A
Transcript:
RefSeq NM_178230.1 (NON-CURRENT)
Match location:
Position 390 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACACAAATGGTTCCCAGTT
Hairpin Sequence:
5'-CCGG-CAACACAAATGGTTCCCAGTT-CTCGAG-AACTGGGAACCATTTGTGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACACAAATGGTTCCCAGTTCTCGAGAACTGGGAACCATTTGTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACACAAATGGTTCCCAGTTCTCGAGAACTGGGAACCATTTGTGTTG-3'

Other clones with same target sequence:

  1. TRCN0000049147